EIF4A1 (Eukaryotic Initiation Factor 4A-I, eIF-4A-I, eIF4A-I, ATP-dependent RNA Helicase eIF4A-1, DDX2A, EIF4A)

eIF4A1 is a 406aa ATP-dependent RNA helicase belonging to the DEAD box helicase family and eukaryotic initiation factor-4A subfamily with a helicase ATP-binding domain and a helicase C-terminal domain. eIF4F eukaryotic translation initiation complex is formed by the association of eIF4A1 with eIF4E and eIF4E kinase (MNK1), eIF4B, eIF4G, PABPC1. It is closely related to eIF4A2. eIF4A1 is a eukaryotic translation initiation factor involved in protein translation:synthesis where it helps in unwinding the secondary structure of RNA in the 5'-UTR of mRNAs, cap recognition, and is necessary for efficient binding mRNA to the 40S small ribosomal subunit, as well as subsequent scanning for the initiator codon. Ubiquitous expression of the enzyme eIF4A1 is seen in most tissues.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.25-1ug/ml\nImmunohistochemistry (formalin fixed paraffin embedded): 5ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nK562 or RAW cell lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

E8949-39P

Size

100ug

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Can, NHP

Form

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Jones, E. et al. Genomics. 53:248-250 (1998). 2. Kukimoto, I. et al. Biochem. Biophys. Res. Commun. 233:844-847 (1997). 3. Nielsen, PJ. et al. EMBO J. 7:2097-2105 (1988).

Additional Info

Recognizes human eIF4A1. Species Crossreactivity: mouse, chimpanzee, monkey, rat, canine and orangutan. Species sequence homology: opossum (94%), zebrafish, pig, and catfish (88%).