eIF4A2 (Eukaryotic Initiation Factor 4A-II, eIF-4A-II, eIF4A-II, ATP-dependent RNA Helicase eIF4A-2, DDX2B, EIF4F)

eIF4A2 is a 407aa ATP-dependent RNA helicase belonging to the DEAD box helicase family. It contains one helicase ATP-binding domain and a helicase C-terminal domain. eIF4A2, a subunit of the eIF4F complex, acts as a protein-synthesis initiation factor and thus plays an important role in the binding of mRNA to the 43S pre-initiation complex of ribosomes. Research is carried out to elucidate the potential role of eIF4A2 in glucose homeostasis and also in type 2 diabetes in the presence of metabolic stress where eIF4A2 controls specific mRNA translation and protein synthesis rate in pancreatic beta-cells. Human eIF4A2 interacts with the NS5B protein of Hepatitis C virus (HCV) and facilitates the genomic RNA synthesis of NS5B protein by unwinding the secondary structure of the HCV genome. It is ubiquitously expressed in most of normal tissues, but in variable amount.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5-1ug/ml\nImmunohistochemistry (formalin fixed paraffin embedded): 5ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHuman and mouse liver lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

E8949-39Y

Size

100ug

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Bov, Prc

Form

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Richter, NJ. et al. J. Biol. Chem. 274:35415-35424 (1999). 2. Sudo, K. et al. Cytogenet. Cell. Genet. 71:385-388 (1995). 3. Nielsen, PJ. et al. Mammalian Genome. 4:185-186 (1993).

Additional Info

Recognizes human eIF4A2. Species Crossreactivity: bovine, mouse, porcine, rat. Species sequence homology: chicken, monkey.