Home  >  Products  >  eIF4A2 (Eukaryotic Initiation Factor 4A-II, eIF-4A-II, eIF4A-II, ATP-dependent RNA Helicase eIF4A-2, DDX2B, EIF4F)
eIF4A2 (Eukaryotic Initiation Factor 4A-II, eIF-4A-II, eIF4A-II, ATP-dependent RNA Helicase eIF4A-2, DDX2B, EIF4F)

eIF4A2 (Eukaryotic Initiation Factor 4A-II, eIF-4A-II, eIF4A-II, ATP-dependent RNA Helicase eIF4A-2, DDX2B, EIF4F)

Cat no: E8949-39Y

Supplier: United States Biological
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eIF4A2 is a 407aa ATP-dependent RNA helicase belonging to the DEAD box helicase family. It contains one helicase ATP-binding domain and a helicase C-terminal domain. eIF4A2, a subunit of the eIF4F complex, acts as a protein-synthesis initiation factor and thus plays an important role in the binding of mRNA to the 43S pre-initiation complex of ribosomes. Research is carried out to elucidate the potential role of eIF4A2 in glucose homeostasis and also in type 2 diabetes in the presence of metabolic stress where eIF4A2 controls specific mRNA translation and protein synthesis rate in pancreatic beta-cells. Human eIF4A2 interacts with the NS5B protein of Hepatitis C virus (HCV) and facilitates the genomic RNA synthesis of NS5B protein by unwinding the secondary structure of the HCV genome. It is ubiquitously expressed in most of normal tissues, but in variable amount. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.5-1ug/ml Immunohistochemistry (formalin fixed paraffin embedded): 5ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Human and mouse liver lysate Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: E8949-39Y
Reactivities: Human, Mouse, Rat, Bovine, Porcine
Hosts: Rabbit
Applications: Immunohistochemistry, Western Blot
Size: 100ug
Form: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified by Protein A affinity chromatography.
References: 1. Richter, NJ. et al. J. Biol. Chem. 274:35415-35424 (1999). 2. Sudo, K. et al. Cytogenet. Cell. Genet. 71:385-388 (1995). 3. Nielsen, PJ. et al. Mammalian Genome. 4:185-186 (1993).
Additional info: Recognizes human eIF4A2. Species Crossreactivity: bovine, mouse, porcine, rat. Species sequence homology: chicken, monkey.

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