EIF4B (Eukaryotic Initiation Factor 4B, Eukaryotic Translation Initiation Factor 4B, hCG_2014609, eIF4B)

eIF4B is a multidomain eukaryotic translation initiation factor with a range of activities. It contains one RRM (RNA recognition) domain, a consensus RNA-binding site (RNP-CS) near the N terminus, and a central domain rich in aspartic acid, arginine, tyrosine, and glycine (DRYG). The DRYG domain of eIF4B helps in self-association and interaction with the p170 subunit of eIF3. eIF4B binds to both mRNA and 18S ribosomal RNA and thus acts as a bridge between the mRNA and the 40S ribosomal subunit during translation initiation. The ATPase and RNA helicase activity of the DEAD box protein eIF4A is stimulated by eIF4B and thus functions in close association with eIF4F, a heterotrimeric protein that binds to mRNA cap structure and unwinds inhibitory secondary structure in the 5' untranslated region of eucaryotic mRNAs in an ATP-dependent manner. Ubiquitous expression is seen in most tissues.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.005-0.1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nK562 and RAW cell lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

E8949-39F2

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Richter, NJ. et al. J. Biol. Chem. 274:35415-35424 (1999). 2. Milburn, SC. et al. EMBO. J. 9:2783-2790 (1990). 3. Methot, N. et al. Molec. Cell. Biol. 16:5328-5334 (1996).

Additional Info

Recognizes human eIF4B. Species Crossreactivity: chimpanzee, mouse, orangutan, rhesus monkey. Species sequence homology: canine, equine, rat.