EIF4E, phosphorylated (Ser209) (Eukaryotic Translation Initiation Factor 4E, eIF4E, eIF-4E, mRNA Cap-binding Protein, eIF-4F 25kD Subunit, EIF4EL1, EIF4F) (APC)

eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. EIF4E is also known to interact with other partners. The interaction with EIF4ENIF1 mediates the import into the nucleus. Nonphosphorylated EIF4EBP1, EIF4EBP2 and EIF4EBP3 compete with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. Rapamycin can attenuate insulin stimulation, mediated by FKBPs.\n\nApplications: \nSuitable for use in ELISA and Dot Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:1,000 \nDot Blot: 1:500\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

E8949-40N-APC

Size

200ul

Applications

ELISA

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to Allophycocyanin (APC).

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1.Rychlik,W., J. Biol. Chem. 262(22), 10434-10437 (1987). 2.Dorfman,J., Genomics 9(4), 785-788 (1991). 3.Pelletier,J., Genomics 10(4), 1079-1082 (1991). 4.Whalen,S.G., J. Biol. Chem. 271(20), 11831-11837 (1996).

Additional Info

Recognizes human EIF4E when phosphorylated at Ser209.