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eIF4E, phosphorylated (Ser209) (Eukaryotic Translation Initiation Factor 4E, eIF4E, eIF-4E, mRNA Cap-binding Protein, eIF-4F 25kD Subunit, EIF4EL1, EIF4F)

Cat no: E8949-40Q

eIF4E, phosphorylated (Ser209) (Eukaryotic Translation Initiation Factor 4E, eIF4E, eIF-4E, mRNA Cap-binding Protein, eIF-4F 25kD Subunit, EIF4EL1, EIF4F)

eIF4E is the smallest subunit of eIF4F, which is a supramolecular complex consisting of three subunits, eIF4E, eIF4A, and eIF4G, and catalysing the initiation of cap-dependent translation with a sequential function. eIF4E binds selectively to the cap structure and is a master switch that controls eukaryotic translation, because the binding of eIF4E to the cap structure is required for initiating the translation of mRNA and is regulated through the association/dissociation of eIF4E with endogenous 4E-binding proteins (4E-BPs). The protein also promotes cellular growth and transformation. The levels of eIF4E are used as prognostic indicators of clinical outcome in human cancers, including breast cancer and head and neck squamous cell carcinomas. Recent studies reveal that eIF4E also acts as a promoter of nucleocytoplasmic transport of specific transcripts.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:500-1:1000\nImmunohistochemistry (paraffin): 1:50-1:100\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nNIH/3T3 cells\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

E8949-40Q

Size

50ug

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Fumi, A. et al. Science Direct. 355:667-672 (2007). 2. Ivan, T. et al. Molecular and Cellular Biology. 23:8992-9002 (2003).

Additional Info

Recognizes human eIF4E, phosphorylated at Ser209. Species Crossreactivity: mouse and rat.

Read more on Supplier website

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA

Hosts

Mouse

Reactivities

Hum, Mouse

More info

Applications

ELISA

Hosts

Mouse

Reactivities

Hum, Mouse

More info

Applications

ELISA

Hosts

Mouse

More info
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