Elastase (HRP)

Elastase, like trypsin and chymotrypsin, is a serine protease that also hydrolyses amides and esters. It is produced in the pancreas as an inactive zymogen, proelastase, and activated in the duodenum by trypsin. Elastase is distinctive in that it acts upon elastin. Because elastin in found in highest concentrations in the elastic fibers of connective tissues, elastase is frequently used to dissociate tissues which contain extensive intercellular fiber networks. For this purpose, it is usually used with other enzymes such as collagenase, trypsin, and chymotrypsin. Elastase is the enzyme of choice for the isolation of Type II cells from the lung. \n\nApplications: \nSuitable for ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nELISA: 1:200-1:14,000. 1ug of Elastase (porcine pancreas) in a standard capture ELISA using ABTS (A0030) as a substrate for 30 minutes at RT. \nWestern Blot: 1:1000-1:5000\nOptimal dilution determined by the researcher.\n\nStorage and Stability: \nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

E2230-25

Size

1mg

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Prc

Form

Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% gentamicin sulfate. Labeled with Horseradish peroxidase (HRP)

P Type

Pab

Purity

Puri?ed by delipidation, fractionation and ion exchange chromatography.

Isotype

IgG

References

Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981.

Additional Info

Recognizes porcine Elastase. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-rabbit serum and purified and partially purified elastase (porcine pancreas).