Elastase, Neutrophil, Recombinant, Mouse

Neutrophil Elastase (ELA2), also known as polymorphonuclear leukocyte elastase, is a serine protease belonging to the chymotrypsin family. Located primarily in the azurophil granules of polymorphonuclear leukocytes, ELA2 function is the degradation of many extracellular matrix macromolecules (1, 2). \nalpha-1 Antitrypsin (Serpin A1) and secretory leukocyte protease inhibitor (SLPI) have been shown to inhibit ELA2 activity (3). This protein may be involved in lung emphysema, cystic fibrosis, the adult respiratory distress syndrome (ARDS), rheumatoid arthritis, tumor invasion and infectious diseases (1). The purified recombinant mouse ELA2 does not contain the last five amino acid residues of the deduced amino acid sequence (NP_056594).\n\nSource:\nMurine myeloma cell line, NSO-derived Ser27-Arg260 with a C-terminal 10-His tag. Accession # NP_056594\n\nSDS-PAGE: \n34kD, Reducing conditions\n\nSpecific Activity: \n~ 500pmoles/min/ug. Measured by its ability to cleave a fluorogenic peptide substrate, MeOSuc-Ala-Ala-Pro-Val-7-amido-4-methylcoumarin (MeOSuc-AAPV-AMC).\n\nStorage and Stability:\nFor long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

E2230-27

Size

10ug

Reactivities

Mouse

Form

Supplied as a liquid in 25mM Tris, 1M sodium chloride, pH 7.5.

Purity

~ 95%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin: (same/less than) 1.0EU/ug (LAL).

References

1. Bieth, J.G., In Handbook of Proteolytic Enzymes. Barrett, A.J., Rawlings, N.D., & Woessner, J.F. eds. pp. 1517-1523 (2004). 2. Owen, C.A., et al., J. Cell Biol. 131: 775-789 (1995). 3. Wiesner, O., et al., FEBS Letters 579: 5305-5312 (2005).

Alternative Names

ELA2, Polymorphonuclear Leukocyte Elastase