Elastase, Porcine

Elastase, like trypsin and chymotrypsin, is a serine protease that also hydrolyses amides and esters. It is produced in the pancreas as an inactive zymogen, proelastase, and activated in the duodenum by trypsin. Elastase is distinctive in that it acts upon elastin. Because elastin in found in highest concentrations in the elastic fibers of connective tissues, elastase is frequently used to dissociate tissues which contain extensive intercellular fiber networks. For this purpose, it is usually used with other enzymes such as collagenase, trypsin, and chymotrypsin. Elastase is the enzyme of choice for the isolation of Type II cells from the lung. \n\nElastase is also found in blood components . The serum enzyme, identical with pancreatic elastase, differs from the elastase of polymorphonuclear leukocytes. It is indicated that the leukocyte enzyme, which is inhibited by alpha1-antitrypsin but not by pancreatic trypsin inhibitor, mediates pathological elastolysis during acute arteritis and pulmonary emphysema. Bieth et al. (1974) indicate elastase involvement in atherosclerosis and acute hemorrhagic pancreatitis.\n\nActivity\n:(same/more than) 3 units per mg protein\nProtein:~100%\nConcentration: ~1mg/ml\nQuality Control: SDS-PAGE\nOptimum pH: 8.5\nExtinction Coefficient: 22\n\nIsoelectric Point: 8.5\n\nUnit Definition:\n1 unit cleaves 1umole N-succinyl-L-alanyl- L-alanyl-L-alanine-p-nitroanilide per minute at 25C, pH 8.\n\nComposition: \nSingle peptide chain of 240 residues and contains 4 disulfide bridges. \n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C. Reconstitute to nominal volume, aliquot and store at 4 degrees C. Reconstituted product is stable for 12 months.. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

E2230

Size

25mg

Form

Supplied as a dialyzed, lyophilized powder.

Purity

2X Crystallized. Suitable for the isolation of Type II lung cells.

References

1. Ardelt, W., (1974) Biochim. Biophys. Acta 341:318. 2. Ardelt, W., (1975) Biochim. Biophys. Acta 393:267. 3. Ascenzi, P., Menegatti, E., et al., (1983) Mol. Cell. Biochem. 56:33. 4. Atlas, D., and Berger, A. Biochem. 11:4719 (1972). 5. Atlas, D., and Berger, A., (1973) Biochem. 12:2573.

Alternative Names

EC=3.4.21.36.; Porcine pancreas