Elastin is the major extracellular matrix protein of large arteries such as the aorta, lung parenchyma, elastic ligaments and skin imparting characteristics of extensibility and elastic recoil. It is also the major matrix protein in some cartilaginous tissues such as ear cartilage, where the functional role of this protein is less evident. It is found throughout the vertebrate kingdom except for primitive fish. Once laid down in tissues, polymeric elastin is able to sustain its mechanical resilience through thousands of millions of cycles of extension and recoil. Elastin is usually synthesized as a monomer, tropoelastin, which is subsequently assembled into a stable, polymeric structure in the extracellular matrix. This polymer is stabilized by covalent cross-links formed through interactions between side chains of lysine residues after oxidative deamination by lysyl oxidase. The protein also consists of 36 domains with alternating hydrophobic and cross-linking characteristics. The hydrophobic domains, predominantly containing glycine, proline, leucine and valine, are responsible for the ability of elastin to align monomeric chains for covalent cross-linking. Defects in elastin are the cause of disorders like autosomal dominant cutis laxa, Williams-Beuren syndrome and supravalvular aortic stenosis.
Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Western Blot: 1:100-1:500
Immunohistochemistry (frozen): 1:50-1:100 (ABC). Incubate primary antibody for 1hr at RT
Immunohistochemistry (formalin fixed paraffin embedded): 1:50-1:100
Optimal dilutions to be determined by the researcher.
Positive Control:
Human lung cell line
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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