ELF3 is a 41-43kD member of the ETS family of proteins. During uncomplicated (non-inflammatory) periods of cell differentiation, ELF3 is expressed exclusively by epithelial cells, repressing genes needed during early differentiation, and promoting genes needed for full differentiation. Under conditions of inflammation, cells such as monocytes, endothelial cells and chrondrocytes express ELF3 and produce molecules such as Ang1 and COX2. Human ELF3 is 371aa in length. It contains one PNT/pointed dimerization domain aa46-132, a protein stabilizing PEST sequence aa210-225, an A/T Hook region that binds to AT-rich DNA sequences aa236-252, and an ETS DNA binding domain aa273-355. ELF3 interacts with CREBBP, EP300, KU70 and KU86. There is one splice variant that shows a deletion of aa174-200. Over aa1-173, human ELF3 shares 87aa identity with mouse ELF3.
Applications:
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution:
Western Blot: 0.1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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