ELISA Assay Diluent (5X)

ELISA Assay Diluent is a 5x concentrated phosphate buffered saline solution containing (pre-screened) bovine serum. This buffer is specifically formulated and tested for ELISA applications, such as blocking antibody-coated ELISA plate microwells, diluting ELISA standards and samples, and diluting biotinylated detection antibodies or tertiary reagents (specifically Avidin-HRP or Streptavidin-HRP). The diluent contains a preservative compatible with peroxidase mediated enzyme activity.

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SPECIFICATIONS

Catalog Number

421203

Size

100 ml

Applications

ELISA

Dilutions

Prior to use, dilute the ELISA Assay Diluent (5X) in PBS, pH 7.4.PBS recipe: 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, and 0.2 g KCl. Add deionized water to 1.0 L, filter through 0.2 microm filter, pH to 7.4

Storage Temp

4 C

Concentration

Shipping Temp

Ambient RT

Storage Buffer

This solution can be stored unopened at 4C for up to the expiration date as labeled. After opening, it is recommended to use within 30 days or to be aliquoted and stored at -20C (frost-free) or -70C for long term storage. Warm ELISA reagents to room temperature (18-25C) before use.

Alternative Names

ELISA assay diluent, ELISA diluent

Regulatory Status

RUO