ELISA Development Kit, BioAssay,(TM) Mouse

ELISA Development Kit, BioAssay,(TM) Mouse is designed to detect and quantitate antigen-specific antibodies in serums and other samples using a proprietary one-hour indirect ELISA procedure. Ideal for large-scale antibody titer determination. This kit allows user to perform a complete ELISA from antigen coating to signal development in just one hour. The one-hour protocol is contrasted with a classical five-hour ELISA procedure.\n\nEasy to Use:\n1. Coat the plate with an antigen (protein or peptide) in the coating buffer for five minutes.\n2. Block the plate with pretreatment solution for five minutes\n3. After the 30-minute binding phase, treat the plate with the ELISA solution for 20 minutes.\n4. The plate can then be developed with the custom TMB solution included.\n\nKit Components:\nThe kit contains all necessary reagents and buffers for performing indirect ELISA using ten 96-well plates.\nDilution Solution, 1x125ml \nELISA Solution, 1x125ml\nTMB, 1x110ml \nPretreat A Solution, 1x125ml\nPretreat B Solution, 1x125ml\nStop Solution, 1x125ml\n20X Wash Solution, 1x125ml\n2X Coating Buffer, 1x 125ml\n\nKey Features:\nFast: This kit cuts indirect ELISA time down to one hour.\nSensitive: Same as or better than standard procedures.\nReproducible: The kit produces highly reproducible results.\nMinimal optimization: Needs significantly less optimization than classical ELISA methods.\n\nStorage and Stability:\nStore the kit at 4 degrees C. It will remain stable for six months. Do not freeze the kit or any of its components.

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