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Elk-1 Control Proteins

Cat no: E2240-20

Elk-1 Control Proteins

The transcription factor Elk-1 is a component of the ternary complex that binds the serum response element (SRE) and mediates gene activity in response to serum and growth factors (1-3). Elk-1 is phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its carboxy terminus; phosphorylation at these sites, particularly Ser383, is critical for transcriptional activation by Elk-1. Elk-1 appears to be a direct target of activated MAP kinase: (a) biochemical studies indicate that Elk-1 is a good substrate for MAP kinase; (b) the kinetics of Elk-1 phosphorylation and activation correlate with MAP kinase activity; (c) interfering mutants of MAP kinase block Elk-1 activation in vivo. Other studies have shown that Elk-1 (Ser383) is also a target of the stress-activated kinase SAPK/JNK (4,5).\n\nMolecular Weight:\n40kD nonphosphorylated, 50kD phosphorylated\n\nNonphosphorylated Elk1 fusion protein: Bacterially expressed GST-Elk1 fusion protein serves as a negative control. Supplied in SDS Sample Buffer. Store at -20 degrees C. \n\nPhosphorylated Elk1 fusion protein: GST-Elk1 phosphorylated by the Erk2 enzyme serves as a positive control. Supplied in SDS Sample Buffer. Store at -20 degrees C.\n\nDirections for Use:\nAs controls, we recommend using 15ul of phosphorylated and nonphosphorylated Elk1 control extracts. Boil sample prior to loading. 150ul wil be sufficient for 10 blots.\n\nWestern Blots: For controls, we recommend using 15ul of phosphorylated and nonphosphorylated Elk1 control extracts. Boil sample prior to loading.

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SPECIFICATIONS

Catalog Number

E2240-20

Size

150ul

Form

Supplied as a liquid in SDS Sample Buffer: 62.5mM Tris-HCl, pH 6.8 at 25 degrees C, 2% w/v SDS, 10% glycerol, 50mM DTT, 0.01% w/v phenol red.

References

1. Marais, R., Wynne, J. and Treisman, R. (1993) Cell 73:381

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