Elk-1

The transcription factor Elk-1 is a component of the ternary complex that binds the serum response element (SRE) and mediates gene activity in response to serum and growth factors (1-3). Elk-1 is phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its C-terminus; phosphorylation at these sites, particularly Ser383, is critical for transcriptional activation by Elk-1. Elk-1 appears to be a direct target of activated MAP kinase: (a) biochemical studies indicate that Elk-1 is a good substrate for MAP kinase; (b) the kinetics of Elk-1 phosphorylation and activation correlate with MAP kinase activity; (c) interfering mutants of MAP kinase block Elk-1 activation in vivo. Other studies have shown that Elk-1 (Ser383) is also a target of the stress-activated kinase SAPK/JNK (4,5).\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution: \nWestern Blot: 1:1000, Incubate membrane with diluted antibody in 5% BSA, 1XTBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight. \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability: \nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

E2240

Size

100ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservatives added.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Marais, R. et al. (1993) Cell 73, 381-393. 2. Kortenjann, M. et al. (1994) Mol. Cell. Biol. 14, 4815-4824. 3. Hill, C.S. and Treisman, R. (1995) Cell 80, 199-211. 4. Cavigelli, M. et al. (1995) EMBO J. 14, 5957-5964. 5. Whitmarsh, A.J. et al. (1995) Science 269, 403-407. 6. Aplin, A.E. et al. (2001) J. Cell Biol. 153, 273-282. 7. Berman, D.E. et al. (1998) J. Neurosci. 18, 10037-10044. 8. Majka, M. et al. (2000) Blood 96, 4142-4151. 9. Stewart, S. and Guan, K.L. (2000) J. Biol. Chem. 275, 8854-8862. 10. Wang, D. and Richmond, A. (2001) J. Biol. Chem. 276, 3650-3659.

Additional Info

Detects endogenous (phosphorylation-state independent) levels of total Elk-1 protein. Species Crossreactivity: Human, mouse and rat.