Elk-1, phosphorylated (Ser383)

Elk-1, a 62kD transcription factor, is the most abundant component of ternary complex factors that is regulated by phosphorylation and in turn regulates gene expression in response to multiple stimuli. Elk-1 is a substrate of MAP kinase and phosphorylated by ERK2 on several sites in the carboxyl terminus, including serine 383. Phosphorylation of Elk-1 on serine 383 is critical for its transcriptional activity, including Elk-1-mediated transcription of c-Fos. Dephosphorylation of serine 383 by calcineurin downregulates Elk-1 transcriptional activity.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000 \nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nY-1 adrenocortical tumor cells; NIH3T3 cells +/- EGF\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

E2240-01A

Size

10 Blots

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide, 50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

1. Espanel, X., et al. (2003) J. Biol. Chem. 278(17):15162-15167. 2. Li, Q.J., et al. (2003) EMBO J. 22(2):281-291. 3. Bardwell, A.J., et al. (2003) Biochem. J. 370(Pt 3):1077-1085. 4. Ahn, Y.M., et al. (2002) Neurosci. Lett. 329(1):9-12. 5. Aplin, A.E., et al. (2001) J. Cell Biol. 153(2):273-282. 6. Tian, J. and M. Karin (1999) J. Biol. Chem. 274(21):15173-15180. 7. Sugimoto, T., et al. (1997) J. Biol. Chem. 272(47):29415-29418.

Additional Info

Recognizes human Elk-1 when phosphorylated at Ser383. Species sequence homology: Mouse (100%).