Elk-1, phosphorylated (Ser383)

Supplier:

United States Biological

Cat no: E2240-01E

Elk-1, phosphorylated (Ser383)

Elk-1, a 62kD transcription factor, is the most abundant component of ternary complex factors that is regulated by phosphorylation and in turn regulates gene expression in response to multiple stimuli. Elk-1 is a substrate of MAP kinase and phosphorylated by ERK2 on several sites in the carboxyl terminus, including serine 383. Phosphorylation of Elk-1 on serine 383 is critical for its transcriptional activity, including Elk-1-mediated transcription of c-Fos. Dephosphorylation of serine 383 by calcineurin downregulates Elk-1 transcriptional activity.\n\nApplications: \nSuitable for use in ELISA. Other applications not tested.\n \nRecommended Dilution:\nIndirect ELISA: 1ug/ml of peptide/or peptide conjugate in pH 9.5 carbonate buffer, 100ul/well.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50?, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

E2240-01E

Size

500ug

Applications

ELISA

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophilized powder from a solution in 0.01M PBS, pH 7.2. Reconstitution: Double distillated water is recommended to adjust the final concentration to 1mg/ml.

P Type

Mab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG1,k

Additional Info

Reactive to the immunogen human Elk-1, phosphorylated [pS383], and peptide-BSA conjugating.

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SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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