Elk-1 is a transcription factor that is part of the ets oncogene superfamily. Elk-1 forms a ternary complex with the serum response factors, SRE and SRF, mediating gene activity in response to serum and growth factors. Activation by several mitogen-activated protein kinases (Erk1 and Erk2) mediates the immediate early responses of the c-fos promoter to growth factors and other stimuli. Serines 383 and 389 in the C-terminal region are the preferred sites for phosphorylation-induced activation.
Applications:
Suitable for use in Immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Immunofluorescence: 1:100-1:200
Immunohistochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.