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Elk1, phosphorylated (Ser389) (ETS Domain-containing Protein Elk-1)

Cat no: E2240-10N

Elk1, phosphorylated (Ser389) (ETS Domain-containing Protein Elk-1)

Elk-1 belongs to the ternary complex (TCF) subfamily of the ETS-domain transcription factor. The protein contains the four functional domains characteristic of all members of the TCF family. The TCFs are major nuclear targets for the RAS-MAPKs of the extracellular-signal regulated kinase (ERK) subfamily, and the closely related SAPK/JNK and p38MAPK stress-activated protein kinases. In vitro, Elk-1 functions as a transcriptional activator via its association with serum response factor (SRF) in a ternary complex on the serum response element (SRE) of many immediate early genes. De-regulation of Elk-1 expression has been associated with cancers of the prostrate and breast, as well as certain T-cell malignancies. Elk-1 is a key player in the integration of mitogenic and stress-mediated signalling pathways. Human Elk-1 gene is mapped to chromosome Xp11.2.\n\nApplications:\nSuitable for use in Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry (Formalin fixed paraffin embedded): 1:50-1:100\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

E2240-10N

Size

50ug

Applications

IHC

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Tanguy, A. et al. Nucleic Acids Research. 35:4649-4663 (2007). 2. Janknecht, R. et al. Oncogene. 9:1273-1278 (1997).

Additional Info

Recognizes human Elk1 (Ser389) when phosphorylated at Ser389. Species Crossreactivity: mouse and rat.

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SUPPLIER INFO

Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info

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