ELMO1 (Engulfment and Cell Motility Protein 1, Protein Ced-12 Homolog, KIAA0281)

ELMO1 protein, initially identified as a mammalian homologue of C. elegans CED-12, is required for cell migration and engulfment of dying cells. The human ELMO1 gene is localized on chromosome 7p14.2 and encodes a polypeptide with 44% similarity to C. elegans CED-12. Multiple transcript variants encoding three different isoforms for the protein has been identified. ELMO1 is a soluble cytoplasmic protein with a potential SH3 binding PxxP motif near the C terminus (conserved from worm to human), a putative leucine zipper around aa630 (conserved in all mammalian proteins) and a putative pleckstrin homology (PH) domain. It is widely expressed in all tissues. The protein functionally cooperates with Crk and dedicator of cytokinesis 1 (Dock180), and promotes phagocytosis and morphological changes.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-2ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nJurkat lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

E2248-02C

Size

100ug

Applications

WB

Hosts

Goat

Reactivities

Hum

Form

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Western blot: Approx 70kDa and 50kDa bands seen in Jurkat cell lysate. Recommended for use at 1-2microg/ml. Note the smaller isoform of this protein has a consensus glycosylation site, which may explain the higher than expected band size (50 kDa versus 30 kDa). Glycosylation can affect migration of proteins on SDS-PAGE [Hames, B.D and Rickwood, D., eds. Gel electrophoresis of proteins: a practical approach. Oxford University Press].

Additional Info

Recognizes human ELMO1. Species sequence homology: mouse.