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ELMO1 (Engulfment and Cell Motility Protein 1, Protein Ced-12 Homolog, KIAA0281)

ELMO1 (Engulfment and Cell Motility Protein 1, Protein Ced-12 Homolog, KIAA0281)

Cat no: E2248-02C

Supplier: United States Biological
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ELMO1 protein, initially identified as a mammalian homologue of C. elegans CED-12, is required for cell migration and engulfment of dying cells. The human ELMO1 gene is localized on chromosome 7p14.2 and encodes a polypeptide with 44% similarity to C. elegans CED-12. Multiple transcript variants encoding three different isoforms for the protein has been identified. ELMO1 is a soluble cytoplasmic protein with a potential SH3 binding PxxP motif near the C terminus (conserved from worm to human), a putative leucine zipper around aa630 (conserved in all mammalian proteins) and a putative pleckstrin homology (PH) domain. It is widely expressed in all tissues. The protein functionally cooperates with Crk and dedicator of cytokinesis 1 (Dock180), and promotes phagocytosis and morphological changes. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Jurkat lysate Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: E2248-02C
Reactivities: Human
Hosts: Goat
Applications: Western Blot
Size: 100ug
Form: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
References: 1. Western blot: Approx 70kDa and 50kDa bands seen in Jurkat cell lysate. Recommended for use at 1-2microg/ml. Note the smaller isoform of this protein has a consensus glycosylation site, which may explain the higher than expected band size (50 kDa versus 30 kDa). Glycosylation can affect migration of proteins on SDS-PAGE [Hames, B.D and Rickwood, D., eds. Gel electrophoresis of proteins: a practical approach. Oxford University Press].
Additional info: Recognizes human ELMO1. Species sequence homology: mouse.

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