ELMO1 (Engulfment and Cell Motility Protein 1, Protein Ced-12 Homolog, KIAA0281)

ELMO1 protein, initially identified as a mammalian homologue of C. elegans CED-12, is required for cell migration and engulfment of dying cells. The human ELMO1 gene is localized on chromosome 7p14.2 and encodes a polypeptide with 44% similarity to C. elegans CED-12. Multiple transcript variants encoding three different isoforms for the protein has been identified. ELMO1 is a soluble cytoplasmic protein with a potential SH3 binding PxxP motif near the C terminus (conserved from worm to human), a putative leucine zipper around aa630 (conserved in all mammalian proteins) and a putative pleckstrin homology (PH) domain. It is widely expressed in all tissues. The protein functionally cooperates with Crk and dedicator of cytokinesis 1 (Dock180), and promotes phagocytosis and morphological changes.\n\nApplications:\nSuitable for use in ELISA, Immunoprecipitation and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:32,000\nWestern Blot: 1-2ug/ml, Jurkat cell lysate observed on ~50, 70kD bands\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

E2248-02D

Size

100ug

Applications

ELISA, IP, WB

Hosts

Goat

Reactivities

Hum, Mouse, Rat, Bov, Can, ZF/Fish

Form

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

1. Epting D, Wendik B, Bennewitz K, Dietz CT, Driever W, Kroll J. The Rac1 regulator ELMO1 controls vascular morphogenesis in zebrafish. Circ Res. 2010 Jul 9;107(1):45-55. 2. Sai J, Raman D, Liu Y, Wikswo J, Richmond A. Parallel phosphatidylinositol 3-kinase (PI3K)-dependent and Src-dependent pathways lead to CXCL8-mediated Rac2 activation and chemotaxis. J Biol Chem. 2008 Sep 26;283(39):26538-47. 3. Jarzynka MJ, Hu B, Hui KM, Bar-Joseph I, Gu W, Hirose T, Haney LB, Ravichandran KS, Nishikawa R, Cheng SY. ELMO1 and Dock180, a bipartite Rac1 guanine nucleotide exchange factor, promote human glioma cell invasion. Cancer Res. 2007 Aug 1;67(15):7203-11. 4. Makino Y, Tsuda M, Ichihara S, Watanabe T, Sakai M, Sawa H, Nagashima K, Hatakeyama S, Tanaka S. Elmo1 inhibits ubiquitylation of Dock180. J Cell Sci. 2006 Mar 1;119(Pt 5):923-32. 5. C

Additional Info

Recognizes human ELMO1. Species Crossreactivity: Bovine and zebrafish. Species sequence homology: Canine, mouse and rat.