Human Elongin A (EloA), otherwise known as transcription elongation factor B polypeptide 3, a general transcription elongation factor, stimulates RNA polymerase II transcription elongation beyond template-encoded arresting sites. Elongin exists as a heterotrimer consisting of an A (A1, A2 or A3), B and C subunit. Subunit A is transcriptionally active, with activity strongly enhanced by binding to the dimeric complex of the elongin regulatory subunits B and C (elongin BC complex), which is mediated by subunit C.
Applications:
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution:
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.