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Embryonic Fibroblasts, P3, Irradiated, Mouse (DR4 MEF)

Cat no: E2256-16

Embryonic Fibroblasts, P3, Irradiated, Mouse (DR4 MEF)

MEF cells serve as feeder cells that support the growth of undifferentiated mouse or human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). MEF cells are isolated from 13.5-day old mouse embryos and should be used at early passages. Before use as feeder cells, MEF cells must be mitotically inactivated by g-irradiation or mitomycin-C treatment.\nDR4 MEF cells are derived from mice that are genetically engineered with 4 drug-resistant genes, neomycin, hygromycin, puromycin and 6-thioguanine.\n\nTreatment:\ngamma-irradiated\n\nCell Type: \nMouse Embryonic fibroblasts\n\nStorage and Stability:\nFor long-term storage, aliquot to avoid repeated freezing and thawing and Store in liquid nitrogen. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for 6 months. \n\nProtocol: \n1. Remove a vial of frozen cells from liquid nitrogen and place it onto dry ice for 5

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SPECIFICATIONS

Catalog Number

E2256-16

Size

5Vials

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