EMP3 (Epithelial Membrane Protein 3)

Epithelial membrane protein 3 (EMP3) is a multipass transmembrane protein in the peripheral myelin protein 22 family. It is expressed as a 20-25kD molecule depending on the degree of glycosylation. EMP3 interacts with the P2X7 purinergic receptor on monocytes. Its dysregulation is associated with glioma and neuroblastoma. Human EMP3 shares 93% aa sequence identity with mouse and rat EMP3.\n\nApplication:\nSuitable for use in Flow Cytometry, Other applications not tested. \n\nRecommended Dilution: \nFlow Cytometry: This antibody was validated for flow cytometry using human PBMC. For intracellular staining to detect EMP3, cells must first be fixed and permeabilized using 4% paraformaldehyde and 0.1% saponin in PBS. Dilute this antibody to 25ug/ml and add 10ul of the diluted solution to 1-5 x 10e5 cells in a total reaction volume not exceeding 200uL. The binding of unlabeled monoclonal antibodies may be visualized by adding a secondary developing reagent such as anti-mouse IgG conjugated to a fluorochrome.\nOptimal dilutions should be determined by each laboratory for each application. \n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol, PBS and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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