ENA78, Recombinant, Human (Epithelial Neutrophil Activating Peptide 78)

Recombinant Human ENA-78 produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 74 amino acids and having a molecular mass of 8020 Dalton. Recombinant Human ENA-78 is purified by proprietary chromatographic techniques.\n\nAmino Acid Sequence: \nThe sequence of the first five N-terminal amino acids was determined and was found to be, Ala- Ala -Val-Leu-Arg. .\n\nDimers and Aggregates: \nLess than 1% as determined by silver-stained SDS-PAGE gel analysis.\n\nBiological Activity: \nRecombinant Human ENA-78 is fully biologically active when compared to standard. The Activity is calculated by the ability to chemoattract Human peripheral blood neutrophils using a concentration of 5-10 ng/ml.\n\nEndotoxin: \n(same/less than) 0.1ng/ug (IEU/ug) of Recombinant Human Epithelial neutrophil-activating protein 78\n\nProtein Content: \nProtein quantitation was carried out by two independent methods:\n1. UV spectroscopy at 280 nm .\n2. Analysis by RP-HPLC, using a calibrated solution of ENA-78 as a Reference Standard.\n\nSolubility: \nIt is recommended to reconstitute the lyophilized ENA-78 in sterile 18MOmega-cm H2O not less than 100microg/ml, which can then be further diluted to other aqueous solutions.\n\nStability: \nLyophilized ENA-78 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution ENA-78 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

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