Endo-beta-N-acetylglucosaminidase F3, Recombinant, Flavobacterium meningosepticum (Endo F3)

Endo-beta-N-acetylglucosaminidase F3 (Endo F3) from Flavobacterium Meningosepticum N-glycans are commonly found on various glycoproteins. While peptide N-glycosidase from Flavobacterium meningosepticum (PNGase F) is widely used to release virtually all types of N-glycans under denaturing conditions, endo-beta-N- acetylglucosaminidases from the same bacterial species can be used under native conditions to specifically release particular types of N-glycans (1, 2). Because these glycosidases hydrolyze the chitobiose core of N-glycans, the released glycan products will contain one GlcNAc residue at their reducing ends with the other GlcNAc residue remaining attached to the asparagine residue on the glycoproteins. Endo F3 hydrolyzes bi- and triantennary N-glycans (3). In general, this enzyme is more efficient on core \nfucosylated N-glycans increasing activity up to 400-fold with biantennary structures (4). It also cleaves fucosylated trimannosyl core structures. \n\nSource:\nRecombinant Flavobacterium meningosepticum Endo-beta-N- acetylglucosaminidase F3 (AAA24924 aa44- 329) was expressed with a 6X-His tag at the N-terminus in E. coli.\n\nMolecular Mass: The purified rFmEndo F3 has the N-terminal amino acid sequence of . The \n293 amino acid residue rFmEndo F3 predicts a molecular mass of 32kD and migrates as a \n30kD band in SDS-PAGE under reducing conditions. \n\nAA Sequence: \nMHHHHHHA44GS\n\nActivity: Measured by its ability to deglycosylate rmTSLP under native conditions. \n\nEndotoxin: (same/less than)1.0 EU/1ug (LAL)\n\nStorage and Stability:\n12 months from date of receipt, 20 to 70 degrees C as supplied.\n1 month, 2 to 8 degrees C under sterile conditions after reconstitution.\n3 months, 20 to 70 degrees C under sterile conditions after reconstitution.\nUse a manual defrost freezer and avoid repeated freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

E2290-11

Size

10ug

Form

Supplied as a lyophilized powder in 0.2um sterile-filtered solution in 25mm Tris, 150mM NaCl, 1mM EDTA, pH7.5 at a concentration of 0.231mg/ml.

Purity

(same/more than) 95% by SDS

References

1. Maley, F., et al., 1989, Anal. Biochem. 180: 195 - 204. 2. Tarentino, A.L., et al., 1985, Biochemistry 24: 4665 - 4671. 3. Tarentino, A.L., et al., 1993, J. Biol. Chem. 268: 9702 - 9708. 4. Tarentino, A.L., and Plummer, T.H. Jr., 1994, Glycobiology 4: 771 - 773.

Additional Info

Recognizes Flavobacterium Endo F3.