Endoglin is a homodimeric integral membrane glycoprotein comprised of 95kD subunit. Endoglin is a component of the TGF-B receptor system that functions as an ancillary receptor influencing binding of the Transforming Growth Factor beta (TGF-B) family ligands to signaling receptors. Endoglin binds TGF-B1 and TGF-B3 with high affinity but does not bind TGF-B2. Endoglin is expressed by vascular endothelial cells and syncytiotrophoblasts of placenta and is weakly expressed by stromalfibroblasts. It is also expressed by U937 cells and activated macrophages. Endoglin expression is up regulated on activated endothelium in tissues undergoing angiogenesis, such as in tumors, or in cases of wound healing or dermal inflammation.
Applications:
Suitable for use in Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilution:
Western Blot: 1:1000
Immunohistochemistry: For use on acetone or paraformaldehyde fixed tissue.
Optimal dilutions to be determined by the researcher.
Positive Control:
Jurkat cell lysate
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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