EndoGlyx-1

EndoGlyx-1 is a glycoprotein complex (~500kD) expressed on normal human blood vessel endothelial cells but not other cells of mesenchymal, neuro- ectodermal, and epithelial derivation. Fetal and some neoplastic endothelial cells express EndoGlyx-1. EndoGlyx-1 is not found in sinusoidal endothelial cells of the spleen and liver. Its expression is not affected after cytokine stimulation.\nEndoGlyx-1 (also designated PEM87 and EMI-3) is an EMILIN-like protein composed of a signal sequence, an N-terminal EMI domain, a C-terminal C1q-like domain, separated from each other by a central coiled-coil-rich region. Four EndoGlyx-1 subunits (p125, p140, p110, and p200) are exposed on the endothelial cell surface.\n\nApplications: \nSuitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Not suitable for use in Western Blot. Other applications have not been tested. \n\nRecommended Dilutions: \nImmunohistochemisty: Frozen sections: unfixed, acetone-fixed or ethanol-fixed. Optimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

E2290-75

Size

100ug

Applications

FC, IHC, IP

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG1

Additional Info

Recognizes human EndoGlyx-1 glycoprotein that is expressed on endothelial cells of human normal and tumor blood vessels, but not on cells of mesenchymal, neuroectodermal and epithelial derivation.