Endomucin (endothelial sialomucin; also Endomucin-1/2 and Mucin-14) is an 80-120kD glycoprotein member of the Endomucin family of proteins. It is expressed on endothelial cells and depending upon its glycosylation pattern, can serve as either a pro- or anti-adhesive molecule. Mouse Endomucin precursor is 261 amino acids in length. It is type I transmembrane protein that contains a 170 aa extracellular domain (ECD) (aa 21-190) and a 50 aa cytoplasmic region. Three splice variants exist in the ECD. One shows a deletion of aa 91-141, a second shows a one aa substitution for aa 91-129, and a third shows a one aa substitution for aa 129-142. Over aa 21-90, mouse Endomucin shares 60% and 30% aa identity with rat and human Endomucin, respectively.
Applications:
Suitable for use in Flow cytometry, Direct ELISA, Western Blot and Immunocytochemistry. Other applications not tested.
Recommended Dilution:
Flow Cytometry: 25ug/mL use 10ul to label 10e6 cells
Direct ELISA: 0.5-1ug/mL detection limit for rmEndomucin ~ 0.3ng/well.
Western Blot: 0.1-0.2ug/mL detection limit for rmEndomucin ~ 1ng/lane under non-reducing and reducing conditions.
Immunocytochemistry: 10ug/mL
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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