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Endonuclease, Hercules

Cat no: E2291-42

Endonuclease, Hercules

Hercules Endonuclease is a engineered endonuclease from Serratia marcescens. The endonuclease attacks and degrades all forms of DNA and RNA (single stranded, double stranded, linear and circular) and is effective over a wide range of operating conditions. The enzyme completely digests nucleic acids to 5'-monophosphate terminated oligonucleotides 2-5 bases in length.\n\nSource: Purified from an E. coli strain expressing a recombinant clone.\n\nApplications:\nViscosity reduction in protein extracts.\nSample preparation for 2D gel electrophoresis.\nRemoval of nucleic acid contaminants from recombinant protein preparations.\n\nUnit Definition: One unit of Hercules Endonuclease is defined as the amount of enzyme that causes an A260 change of 1.0 in 30 minutes at 37 degrees C, which corresponds to complete digestion of 37ug of DNA.\n\nStorage & Stability:\nStable at 4 degrees C for several weeks, for long-term storage, store it at -20 degrees C after receiving. No obvious decrease of enzyme activity can be found after an incubation at 37 degrees C for a week.

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SPECIFICATIONS

Catalog Number

E2291-42

Size

25KU

Form

Storage Buffer: 10mM Tris-HCl, pH8.0, 2mM MgCl2, 20mM NaCl, 50% glycerol.

Purity

(same/more than) 99% as determined by SDS-PAGE.

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Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA

Hosts

Mouse

Reactivities

Hum, Mouse

More info

Applications

ELISA

Hosts

Mouse

Reactivities

Hum, Mouse

More info

Applications

ELISA

Hosts

Mouse

More info
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