Endophilin, pan, SH3 Domain

Endophilin-3 is a 40kD protein that shows more ubiquitous expression than its counterparts endophilin-1 (40kD), which is preponderant in the brain, and endophilin-2 (45kD) that is found in the brain as well as the testis. Endophilins (1, 2 and 3) have a domain structure similar to amphiphysin with a conserved amino-terminal domain and a carboxy-terminal SH3\ndomain. Endophilins have been proposed to play roles in membrane trafficking reactions through interactions with endocytic proteins via physical or chemical modifications of membrane bilayers, particularly in presynaptic vesicle trafficking at nerve terminals.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications have not been tested.\n\nRecommended Dilution:\nELISA: 0.1-1ug/ml\nWestern Blot: 1-3ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

E2291-48

Size

100ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Mouse

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

P Type

Pab

Purity

Purified by epitope affinity chromatography.

Additional Info

Reacts with mouse endophilin 1, 2 and 3 proteins. Based on 100% sequence homology, it is expected to cross-react with human and rat samples. On Western blots of mouse brain homogenates, two bands at ~40kD (endophilin 1, 2) and one band at ~45kD (endophilin 3) are identified. A faint band at ~52-55kD is also observed, which may represent an isomer. Reactivity has been confirmed with mouse brain lysates.