Endoproteinase Lys-X (Lysyl Endopeptidase), Mass Spectrometry Grade

Lysyl Endopeptidase is a protease secreted by the soil bacterium Achromobacter lyticus M497-1, and is synonymous with the achromobacter protease discovered by Masaki, et al. Lysyl Endopeptidase cleaves peptide bonds at the carboxy-terminal of both lysine residues and S-aminoethylcysteine residues with a high degree of specificity, making it a valuable tool for analysis of peptide and protein primary structure and for enzymatic synthesis of Lys-X compounds. An added feature of Lysyl Endopeptidase is its ability to retain complete activity after incubation in 4M urea or in 0.1% SDS solution for up to 6 hours at 30 degrees C.\n\nAmong the most important techniques in proteome analyses is the in-gel digestion of protein spots/bands that have been resolved by electrophoresis using digestive enzymes, such as trypsin and lysyl endopoptidase. Proteins can be identified by mass spectrometry analysis of the peptides produced by in-gel digestion, and further information regarding post-translational modifications can be obtained. Lysyl Endopeptidase, Mass Spectrometry Grade is a freeze dried product that has sufficient activity for in-gel digestion and packed in very small quantities for convenience purposes.\n\nMolecular Weight: \n27,000 (gel filtration); 30,000 (SDS electrophoresis)\n\nUnit Definition: 1AU: Amidase Unit is the amount of enzyme required for the formation of 1umol of p-nitroaniline from N-benzoyl-DL-lysine-p-nitroaniline per minute at 30 degrees C, pH 9.5.\n\nActivity:\n0.03-0.07 AU/vial\n\nOptimal pH: 9.0-9.5 (amidase activity)\n\nApplications: \nSuitable for use in Sequencing. Other applications not tested.\n\nIsoelectric Point: 6.9-7.0\n\nStorage and Stability: \nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with 2mmol/L Tris-HCl, pH 8.0. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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