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Endostatin (ES) BioAssay(TM) ELISA Kit (Mouse)

Cat no: 024795

Endostatin (ES) BioAssay(TM) ELISA Kit (Mouse)

Endostatin (ES) BioAssay(TM) ELISA Kit (Mouse) is a sandwich enzyme immunoassay for in vitro quantitative measurement of ES in mouse serum, plasma and other biological fluids.\n\nDetection Range:\n0.156-10ng/ml\n\nSensitivity:\n0.056ng/ml\n\nIntra-Assay CV:\n< 10% \n\nInter-Assay CV:\n< 12%\n\nKit Components:\n*024795A: Microtiter Strip, 1x96 wells, Pre-coated, ready to use\n*024795B: Standard, 2x1 vial\n024795C: Standard Diluent, 1x20ml\n*024795D: Detection Reagent A, 1x120ul\n*024795E: Detection Reagent B, 1x120ul\n024795F: Assay Diluent A, 1x12ml\n024795G: Assay Diluent B, 1x12ml\n024795H: TMB Substrate, 1x9ml\n*024795J: Positive Control, 1x1vial\n024795K: Stop Solution, 1x6ml\n024795L: Wash Buffer, 30X, 1x20ml\n\nPrecaution:\nThe Stop Solution (024795K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.\n\nStorage and Stability:\nStore *024795A, *024795B, *024795D, *024795E and *024795J at -20 degrees C. Store all the other components at 4 degrees C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.\n\nAssay Procedure Summary:\n1. Prepare all reagents, samples and standards;\n2. Add 100ul standard or sample to each well. Incubate 2 hours at 37 degrees C.\n3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37 degrees C.\n4. Aspirate and wash 3 times.\n5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37 degrees C.\n6. Aspirate and wash 5 times.\n7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37 degrees C.\n8. Add 50ul Stop Solution. Read at 450nm immediately.

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SPECIFICATIONS

Catalog Number

024795

Size

96Tests

Additional Info

Mouse

Read more on Supplier website

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

More info

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

More info
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