Endothelial Lipase (EL)

Endothelial lipase (EL) is a member of the triglyceride lipase gene family. It functions primarily as a phospholipase and has low triglyceride lipase activity. It has been shown to be a major genetic determinant for the concentration, structure, and metabolism of high-density lipoprotein, which protects against atherosclerosis (1,2). It was originally cloned from endothelial cells and was found to be expressed in a distinct and complementary tissue-restricted fashion, with high-level expression in the liver, placenta, lung, ovary, and macrophage(3). The wide spread distribution of this protein suggests that it plays a general role in lipid metabolism. Immunohistochemical studies demonstrate that EL is expressed in infiltrating cells such as macrophages within atheromatous plaques, in addition to endothelial and smooth muscle cells in non-atherosclerotic coronary arteries. Furthermore, EL expression is detected in the neovasculature within atheromatous plaques in atherosclerotic coronary arteries, indicating that EL may have unique functional roles in atherosclerosis. \n\nApplications: \nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 3ug/ml\nImmunohistochemistry (FFPE): 2ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nWestern Blot: HepG2 cell lysate\nImmunohistochemistry: Paraffin-embedded rat kidney sections. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

E3010-36

Size

500ul

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Prc, Sheep

Form

Supplied as a liquid in TBS, pH 7.4, 0.5mg/ml BSA, 0.02% sodium azide, 50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography

Isotype

IgG

References

1 Ishida, T., Choi, S., Kundu, R.K., et al., J. Clin. Invest. 111(3): 347-355 (2003). 2. Ma, K., Cilingiroglu, M., Otvos, J.D., et al., Proc. Natl. Acad. Sci. USA 100(5): 2748-2753 (2003). 3. Hirata, K., Dichek, H.L., Cioffi, J.A., et al., J. Biol. Chem. 274(20): 14,170-14,175 (2003). 4. Azumi, H., Hirata, K., Ishida, T., et al., Cardiovascular Res. 58: 647-654 (2003).

Additional Info

Recognizes human Endothelial Lipase at ~57kD. \nSpecies Crossreactivity: mouse, rat, sheep and porcine.