Description:
EPOC growth medium was designed and tested for our human endothelium progenitor Outgrowth cells (EPOC). This is the EPOC growth medium supplement component for E3010-68G. The EPC growth medium supplement includes fetal bovine serum and human recombinant growth factors, and some lot-to-lot variability is unavoidable.
Each lot of this product is tested in cell culture to ensure its sterility. This product does not contain antibiotics.
Use of EPOC Growth Medium for Culturing EPOC:
1. Thaw the supplement and add the entire volume (25ml) to the basal medium (500ml). Antibiotics may also be added if contamination is a concern.
2. Take the amount of EPOC growth medium needed and warm to 37 degrees C in a water bath or incubator. Avoid frequent temperature change to the entire bottle of medium. The EPOC growth medium is stable for at least 1 month stored at 2 -8 degrees C.
3. Refer to manual for EPOC culture procedures using the EPOC growth medium. We recommend changing medium every 2 days for regular cell culture.
Storage and Stability:
Stored at -20 degrees C. If taking aliquots from the original bottle is necessary, dispense the contents and re-freeze in small aliquots to avoid repeated freezing-thawing cycles. Stable for at least 1 year from the date of receipt under proper storage condition.
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