Mouse EPOC growth medium was designed and tested for E3010-71 Mouse endothelium progenitor Outgrowth cells (mEPOC).
This medium requires two components: Mouse EPOC Basal Medium (E3010-72G1) and Mouse EPOC Growth Medium Supplement (E3010-72G2).
E3010-72G1 is the Mouse EPOC Basal Medium component for E3010-72G: Endothelial Progenitor Outgrowth Cells, Mouse, Growth Medium Kit, BioAssay(TM).
Use of EPC Growth Medium for Culturing EPOC:
1. Thaw the supplement (E3010-72G2) and add the entire volume (25ml) to the basal medium (500ml) (E3010-72G1). Antibiotics may also be added if contamination is a concern.
2. Take the amount of EPOC growth medium needed and warm to 37 degrees C in a water bath or incubator. Avoid frequent temperature change to the entire prepared bottle of medium. The prepared Mouse EPC growth medium is stable for at least 1 month stored at 4 degrees C.
Storage and Stability:
Store at 4 degrees C. A change in color or the appearance of a precipitate may indicate deterioration. Stable for 6 months from the date of receipt under proper storage conditions
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