Endothelin A Receptor, aa410-422 (ET-A, ET1-Specific Type Endothelin Receptor, Ednra, ETA, ETRA)

Endothelins are a family of peptides that are potent vasoconstrictors produced by mammalian vascular endothelial cells. The receptors for endothelins are widely expressed on tissues. This sheep antibody recognizes epitopes on the cytoplasmic region of the ET(A) Receptor. The binding of this antibody does not interfere with the function of the receptor so that endothelins can interact with the receptor while the antibody is present.\n\nCellular Localization: Plasma membrane\n\nApplications: \nSuitable for use in Immunofluorescence, Immunoprecipitation, Immunohistochemistry (paraffin-embedded sections) and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

E3100-01A

Size

125ug

Applications

ICC, IF, IHC, IP

Hosts

Sheep

Reactivities

Hum, Mouse, Sheep

Form

Supplied as a liquid in PBS, pH 7.2.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Wulfing P, et al., (2004) Oncol Rep. Apr;11(4):791-6. 2 Siren AL, et al., (2000) Neurochem Res. Jul;25(7):957-69. 3 Shraga-Levine Z, et al., (2000) Cell Mol Neurobiol. Jun;20(3):305-17. 4 Fernandes LB, et al., (1998) J Cardiovasc Pharmacol. 31(Suppl1):S222-4. 5 Barber DA, et al., (1996) Am J Physiol. Jan;270(1 Pt 2):H65-71.

Additional Info

This antibody recognizes epitopes on the cytoplasmic side of the receptor and will not block the binding of endothelins. Therefore, the cell membrane must be permeable to the antibody. Species Crossreactivity: Crossreacts with Human, Mouse and Sheep.