Endothelins are a family of peptides that are potent vasoconstrictors produced by mammalian vascular endothelial cells. The receptors for the endothelins are found on various tissues including, but not restricted to, lung and intestine. Endothelin A is free to bind to the receptor even if the antibody is present on the receptor. Tested using Endothelin A receptor transfected COS cells coded for expression of the 39kD segment of the cytoplasmic region of the receptor.
Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions:
Western Blot (ECL): 1:200 on rat brain membranes
Immunohistochemistry: Rat brain sections
Optimal dilutions to be determined by the researcher.
Included Control Antigen:
E3099-91B1: Endothelin A Receptor (aa413-426), Control Antigen
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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