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Endothelium

Cat no: E2292-01J


Supplier: United States Biological
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Applications: Suitable for use in Flow Cytometry, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: 1:20 works well on acetone fixed or unfixed cryostat sections, and periodate/lysine/paraformaldehyde-fixed sections, in which membrane antigens and the structures of vessels and adjacent tissues are optimally preserved. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: E2292-01J
Reactivities: Human, Feline, Guinea Pig
Hosts: Mouse
Applications: Flow Cytometry, Immunocytochemistry, Immunohistochemistry
Size: 1ml
Form: Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein G affinity chromatography.
References: 1. Cui, Y.C., et al., 1983, Immunology 49, 183. 2. Holden, C.A., et al., 1986, J. Immunol. Methods 91, 45. 3. Jones, R.R., et al., 1986, J. Clin. Pathol. 39, 742. 5. Burgio, V.L., et al., 1994, Clin. Exp. Immunol. 96, 170-176.
Additional info: Recognized the human Endothelium. Stains all human blood vessels including brain microvessels. Both large and small vessels are equally reactive. Strongly stains murine fibroblasts transfected with the human CD31 gene. On surface-iodinated Jurkat T cells, recognizes the 130kD CD31 antigen. No other structures in the skin, heart, kidney, tonsils or spleen are stained. Species Crossreactivity: Guinea-pig, feline. Does not react with rabbit, bovine, sheep, dog, rat or mice endothelial cells.

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