Enkephalin Leu5

Enkephalin and enkephalin-like peptides have been detected in many animal species. In mammals these opioid pentapeptides are cleaved from a common precursor molecule which is expressed in a number of cell types, including neurons and endocrine cells associated with the sympathetic nervous system where they are often co-stored with catecholamines. They are known to be endogenous ligands for Sopioid receptors and are known to mediate analgesia at the spinal level.\n\nApplications:\nSuitable for use in Immunohistochemistry and Immunocytochemistry. Other applications have not been tested.\n\nRecommended Dilutions:\nImmunohistochemistry: 1:100-1:400. Cryostat sections of rat and guinea pig brain stems.\nImmunocytochemistry: 1:300. Primary cultures of rat striatal and hippocampal neurons using Cy2 or Cy3 conjugated secondary antibody for detection.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

E3220-09

Size

100ul

Applications

ICC, IHC

Hosts

Mouse

Reactivities

Rat, GP, Rabbit

Form

Supplied as a liquid in PBS, <0.1% thimerosal.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. (1986) Neurochemistry: Modern Methods and Applications, 49

Additional Info

Recognizes Met5-and Leu5-enkephalin. Displays about 40% crossreactivity with C-terminal extended Met-enkephalin hexapeptides and 7% crossreactivity with the extended heptapeptide (-Arg-Phe-OH) but does not recognize other endogenous peptides. By Immunohistochemistry no crossreactivity to beta-endorphin or dynorphin. Species Crossreactivity: rat, guinea pig, hamster and rabbit.