eNOS KO Mouse Thymus Fibroblasts from�Creative Bioarray�are isolated from thymus tissue of pathogen-free laboratory mice. eNOS KO Mouse Thymus Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in�Creative BioarrayCulture Complete Growth Medium generally for 3-7 days. Cultures are then expanded.�Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6cells per ml and is delivered frozen.