eNOS Phosphorylated (Ser1179) (NOS III, Nitric Oxide Synthase 3, Nitric Oxide Synthase 3 Endothelial Cell, NOS Type III, NOS3, Endothelial Nitric Oxide Synthase 3, Endothelial NOS, eNOS, ECNOS, EC-NOS, Constitutive NOS, cNOS, Nitric Oxide Synthase Endothe

The oxidation of L-arginine by a family of nitric oxide synthase (NOS) isoenzymes, nNOS, eNOS, and iNOS, is responsible for the production of nitric oxide (NO). In mammalian genomes, three separate genes encode the NOS isoforms, two expressed constitutively (nNOS, eNOS), and one inducible (iNOS). Endothelial NOS (eNOS, NOS III) is expressed in endothelial cells, some neurons and cardiac myocytes, and is responsible for cardiovascular homeostatic processes, including blood pressure regulation, vessel remodeling and angiogenesis (1).\neNOS undergoes a unique translocation cycle between the plasma membrane and the cytoplasm, suggesting that its intracellular localization is important in linking stimuli to eNOS enzymatic activity. Phosphorylation of the protein at several sites also plays a regulatory role. Phosphorylation of eNOS at Ser1179 in response to stimulation by VEGF has been observed to enhance eNOS activity, increasing the production of NO (1), while phosphorylation at Thr497 in combination with dephosphorylation at Ser1179 inhibits eNOS activity (2). Ser1179 (bovine)/Ser1177 (human) is the target residue for phosphorylation of multiple protein kinases, including Akt (3), AMP kinase (4), PKA and protein kinase G (5), and CaM kinase II (6).\n\nApplications: \nSuitable for use in Immunofluorescence, ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nELISA: 0.1-1ug/ml\nWestern Blot: 1-3ug/ml\nImmuno?uorescence: Reactive with monkey COS cells (1) \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

N5350-30

Size

50ug

Applications

ELISA, IF, WB

Hosts

Rabbit

Reactivities

Bov

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Fulton D, et al. J Biol Chem 277(6):4277-4284, (2002). 2. Michell BJ, et al. J Biol Chem 277(44):43244-42351, (2002). 3. Dimmeler S, et al. Nature 399(6736):601-605, (1999). 4. Chen ZP, et al. FEBS Lett 443(3):285-289, (1999). 5. Butt E, et al. J Biol Chem 275(7):5179-5187, (2000). 6. Fleming I, et al. Circ Res 88:e68-e75, (2001).

Additional Info

Recognizes bovine eNOS protein, phosphorylated at Ser1179 (~135kD). Reactivity has been con?rmed with VEGF-stimulated, serum-starved BAEC (bovine aortic endothelial cell) lysates. A very low signal was observed in the lysates of serum-starved, unstimulated BAEC and no signal was observed in lysates of unstimulated BAEC in the presence of serum. Species Sequence Homology: human, rat, mouse, canine, porcine and guinea pig