eNOS, phosphorylated (Thr495) (Endothelial Nitric Oxide Synthase, NOS 3, NOS III)

Endothelial nitric-oxide synthase (eNOS) is an important enzyme in the cardiovascular system. It catalyzes the production of nitric oxide (NO), a key regulator of blood pressure, vascular remodeling and angiogenesis (1,2). eNOS is regulated by phosphorylation at multiple sites. The two most throughly studied sites are the activation site serine 1177 and the inhibitory site threonine 495 (3). Several protein kinases including Akt/PKB, PKA and AMPK activate eNOS by phosphorylating Ser1177 in response to various stimuli (4,5). In contrast, bradykinin and hydrogen peroxide activate eNOS activity by promoting Thr495 dephosphorylation (6,7).\n\nApplications: \nSuitable for use in Immunoprecipitation and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nImmunoprecipitation: 1:100\nWestern Blot: 1:1000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

N5350-31

Size

100ul

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

P Type

Pab

Purity

Purified by Protein A and peptide affinity chromatography.

Isotype

IgG

References

1. Fulton, D., et al., J. Pharmacol. Exp. Ther. 299: 818

Additional Info

Recognizes endogenous levels of eNOS only when phosphorylated at threonine 495. Species Crossreactivity: Bovine and porcine. Reactivity is expected with human, mouse and rat due to 100% sequence homology with peptide used for immunogen.