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Entactin (Nidogen 1)

Entactin (Nidogen 1)

Cat no: E3226-05A

Supplier: United States Biological
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Nidogen 1 / Entactin is a sulfated glycoprotein which is widely distributed in basement membranes and that is tightly associated with laminin. Also binds to collagen IV. It probably has a role in cell extracellular matrix interactions. Applications: Suitable for use in ELISA, Western Blot, and Immunohistochemistry (frozen). Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Reconstitute with sterile 40-50% glycerol, 500ul ddH2O, aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: E3226-05A
Reactivities: Mouse
Hosts: Rabbit
Applications: ELISA, Immunohistochemistry, Western Blot
Size: 20ul
Form: Supplied as a lyophilized powder. Reconstitute with 20ul ddH2O.
P type: Pab
Purity: Serum
References: 1. Salmivirta, K., et al., (2002), "Binding of mouse nidogen-2 to basement membrane components and cells and its expression in embryonic and adult tissues suggest complementary functions of the two nidogens", Exp. Cell Res., 279:188-201. 2. Kohfeldt, E., et al., (1998), "Nidogen-2: a new basement membrane protein with diverse binding properties", J. Mol. Biol., 282:99-109. 3. Schymeinsky, J., et al., (2002), "Gene structure and functional analysis of the mouse nidogen-2 gene: nidogen-2 is not essential for basement membrane formation in mice", Mol. Cell Biol., 22:6820-6830.
Additional info: Recognizes mouse Nidogen 1.

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