Enterovirus

Enterovirus primarily infects the alimentary tract and probably infects other organs through the blood vascular systems. This antibody recognizes most Enterovirus and poliovirus strains and some echovirus in tissue culture preparations.\n\nApplications: \nSuitable for use in Immunofluorescence, Western Blot and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution: \nImmunofluorescence: 1:10\nWestern Blot: 1:100-1:200\nImmunocytochemistry: 1:20-1:40 for 30 min. at RT\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nImmunofluorescence: Acetone-fixed HEp 2 cells infected with poliovirus type 1 (cytoplasmic staining pattern)\nWestern Blot: Sup T cells\nImmunocytochemistry: Formalin-fixed, paraffin-embedded HEp 2 cells infected with poliovirus type 1 (cytoplasmic staining pattern)\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

E3310-01B

Size

1ml

Applications

ICC, IF, WB

Hosts

Mouse

Form

Supplied as a lyophilized powder in 15mM sodium azide. Reconstitute with 1ml dH2O.

P Type

Mab

Purity

Supernatant

Isotype

IgG2a

References

1.Trabelsi A, Grattard F, Nejmeddine, et al.. Evaluation of an enterovirus group-specific anti-VP1 monoclonal antibody, 5-D8/1, in comparison with neu- tralization and PCR for rapid identification of enteroviruses in cell culture. Journal of Microbiology. 33:2454-2457 (1995). 2.Yousef G E, Bell E J, Mann G F, et al.. Chronic enterovirus infection in patients with postviral fatigue syndrome. The Lancet. i:146-150 (1988). 3.Yousef G E, Brown I N, and Mowbray J F. Derivation and biochemical characterization of an enterovirus group-specific monoclonal antibody. Intervirology. 28:163-170 (1987). 4.Yousef G E, Mann G F, Brown I N, et al.. Clinical and research application of an enterovirus group-reactive monoclonal antibody. Intervirology. 28:199- 205 (1987).

Additional Info

Recognizes Enterovirus VP1 peptide. Recognizes most Enterovirus and poliovirus strains and some echovirus in tissue culture preparations.