Enterovirus

Cat no: E3310-01B

Supplier: United States Biological

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Description
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Enterovirus primarily infects the alimentary tract and probably infects other organs through the blood vascular systems. This antibody recognizes most Enterovirus and poliovirus strains and some echovirus in tissue culture preparations. Applications: Suitable for use in Immunofluorescence, Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilution: Immunofluorescence: 1:10 Western Blot: 1:100-1:200 Immunocytochemistry: 1:20-1:40 for 30 min. at RT Optimal dilutions to be determined by the researcher. Positive Control: Immunofluorescence: Acetone-fixed HEp 2 cells infected with poliovirus type 1 (cytoplasmic staining pattern) Western Blot: Sup T cells Immunocytochemistry: Formalin-fixed, paraffin-embedded HEp 2 cells infected with poliovirus type 1 (cytoplasmic staining pattern) Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Catalogue number:	E3310-01B
Hosts:	Mouse
Applications:	Immunocytochemistry, Immunofluorescence, Western Blot
Size:	1ml
Form:	Supplied as a lyophilized powder in 15mM sodium azide. Reconstitute with 1ml dH2O.
P type:	Mab
Isotype:	IgG2a
Purity:	Supernatant
References:	1.Trabelsi A, Grattard F, Nejmeddine, et al.. Evaluation of an enterovirus group-specific anti-VP1 monoclonal antibody, 5-D8/1, in comparison with neu- tralization and PCR for rapid identification of enteroviruses in cell culture. Journal of Microbiology. 33:2454-2457 (1995). 2.Yousef G E, Bell E J, Mann G F, et al.. Chronic enterovirus infection in patients with postviral fatigue syndrome. The Lancet. i:146-150 (1988). 3.Yousef G E, Brown I N, and Mowbray J F. Derivation and biochemical characterization of an enterovirus group-specific monoclonal antibody. Intervirology. 28:163-170 (1987). 4.Yousef G E, Mann G F, Brown I N, et al.. Clinical and research application of an enterovirus group-reactive monoclonal antibody. Intervirology. 28:199- 205 (1987).
Additional info:	Recognizes Enterovirus VP1 peptide. Recognizes most Enterovirus and poliovirus strains and some echovirus in tissue culture preparations.

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Enterovirus

Cat no: E3310-01B

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