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Envoplakin

Cat no: E3317

Envoplakin

Envoplakin is a precursor of epidermal cornified envelop under the plasma membrane of keratinocytes in the outer most layers of epidermis. It is expressed in keratinizing and nonkeratinizing stratified squamous epithelia but not in simple epithelia and non-epithelia. Loss of envoplakin function has been linked to the development of palmoplanter keratosis which is associated with the increased risk of esophegeal cancers.\n\nApplications:\nSuitable for use in Immunohistochemistry. Other applications have not been tested.\n\nRecommended Dilution:\nImmunohistochemistry (Formalin/paraffin): 1:5 for 60 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 8.0, for 10-20 min followed by cooling at RT for 20 min. Detection may require use of a high-sensitivity detection system.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nSkin\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

E3317

Size

250ul

Applications

ELISA, IHC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, before the addition of glycerol to 40%.

P Type

Mab

Purity

Tissue culture supernatant

Isotype

IgG1

References

1. Ruhrberg, C., et al., (1996) J. Cell Biol. 134:715-729. 2. Ruhrberg, C., et al., (1996) Genomics 37:381-385.

Additional Info

Recognizes human Envoplakin. The epitope is located at the N-terminus.

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Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info

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