Home  >  Products  >  EOMES, NT (Eomesodermin Homolog, T-box Brain Protein 2, TBR2, T-brain-2, TBR-2)
EOMES, NT (Eomesodermin Homolog, T-box Brain Protein 2, TBR2, T-brain-2, TBR-2)

EOMES, NT (Eomesodermin Homolog, T-box Brain Protein 2, TBR2, T-brain-2, TBR-2)

Cat no: E3321

Supplier: United States Biological
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Tbr2 (a T-domain transcription factor) is a member of a conserved protein family that shares a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. Trb2 has been shown to play a role in the development of the neocortex and functions in the differentiation pathway of radial glia to postmitotic projection neurons. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.5ug/ml detected Tbr2 in 10ug of E13-14 mouse brain lysates. Immunohistochemistry (Paraffin): 1:100 detected Tbr2 in mouse and human brain. Requires HIER with citrate buffer pH 6.0 Optimal dilutions to be determined by the researcher. Positive Control: E13-14 mouse brain lysate Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: E3321
Reactivities: Human, Mouse, Rat
Hosts: Chicken/Bird
Applications: Immunohistochemistry, Western Blot
Size: 100ug
Form: Supplied as a liquid in PBS, 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
P type: Pab
Isotype: IgY
Purity: Purified by affinity chromatography.
Additional info: Recognizes mouse Tbr2 at ~73kD. Species Crossreactivity: human and rat.

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