Eosinophil Major Basic Protein (Eosinophil Granule Major Basic Protein, EMBP, MBP, Pregnancy-associated Major Basic Protein)

E3330-01D is a very useful tool for clinical and experimental allergy studies. BMK-13 is a pan-eosinophil marker in both frozen sections and paraffin-embedded tissues. It stains in bronchial sections, 98% of the peripheral blood eosinophils. Further, the antibody can be used in specific ELISA tests to determine the release of MBP and to quantify the number of eosinophils in various in vitro adhesion assays. This antibody is suitable for blood smears (cytospins) if fixed with methanol-acetone mixture (1:1, v/v). It is unlikely, without permeabilization, that the BMK-13 is suitable for the flow cytometry because BMK-13 stains intracellular, stored proteins.\n\nApplications: \nSuitable for use in ELISA, Immunocytochemistry and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry (Frozen): 1:30 diluted in PBS using the APAAP method. Incubate sections for 1 hour at RT. \nImmunohistochemistry (Paraffin): 1:30 diluted in PBS. Incubate sections for 1 hour at RT. Enzymatic pretreatment for paraffin sections is necessary. Pepsin is recommended. Do not use heat denaturation.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

E3330-01D

Size

1ml

Applications

ELISA, ICC, IHC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.

P Type

Mab

Purity

Purified

Isotype

IgG1

References

1. Barkans, J.R., et al., Clin. & Exp. Allergy 20(1): 1 (1990). 2. Moqbel, R., et al., Clin. & Exp. Allergy 22: 265-273 (1992). 3. Spry, C.J.F., et al., Immunology Today 13: 384-387 (1992).

Additional Info

Recognizes the human 10kD Eosinophil Major Basic Protein (MBP) of both resting and activated eosinophils. Crossreacts weakly with human basophils, which also contain low level of this protein. Does not crossreact with any other human protein or cell.