Eotaxin 1

Eotaxin is a 74 amino acid, eosinophil chemotactic CC chemokine originally found in bronchoalveolar lavage fluid from allergic inflammatory subjects. It is involved in regulating the recruitment and activation of inflammatory leukocytes, particularly eosinophils. Eotaxin binds and activates the CCR3 chemokine receptor, and may play a fundamental role in the development of allergic responses.\n\nApplications:\nDirect ELISA\n\nRecommended Dilution:\nDirect ELISA: 1:10,000 dilution will yield 0.4 O.D. using alkaline phosphatase conjugated rabbit anti-mouse Ig\n\nReconstitution:\nReconstitute with dH2O. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. \n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

E3351-01A

Size

500ug

Applications

ELISA

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophilized powder in PBS, pH 7.2.

P Type

Mab

Purity

Purified by ion exchange chromatography.

Isotype

IgG1

References

1. Miller JD, Pegelow DF, Dempsey JA, (2005) J Physiol Jan 13 2. Deheyn DD, Gendreau P, Latz MI, (2005) Mar Environ Res Jul;60(1):1-33. 3. Sosnowski M, Clark E, Macfarlane PW, (2005) Ann Noninvasive Electrocardiol Jan;10(1):7-15. 4. Zinkstok RT, Witte S, Eikema KS, (2005) Opt Lett Jan 1;30(1):78-80. 5. Schlatter A, Krainer L, Paschotta R, (2005) Opt Lett Jan 1;30(1):44-6. 6. Fernandes FC, van Spaendonck RL, (2005) IEEE Trans Image Process Jan;14(1):110-24.

Additional Info

Recognizes human Eotaxin-1.