Eotaxin 2 (MPIF-2,Myeloid progenitor inhibitory factor-2, Chemokine-beta-6, Ck-beta-6, SCYA24, small inducible cytokine A24, CCL24)

This protein of 78 amino acids belongs to the group of cytokines known as CC-Chemokines. Eotaxin-2 is identical with MPIF-2 (Myeloid progenitor inhibitory factor-2) and is known also as Chemokine-beta-6 (abbr. Ck-beta-6). The new designation is SCYA24 [small inducible cytokine A24]. The factor has been renamed CCL24. See also: SCY family of cytokines for a systematic nomenclature. The eotaxin-2 and eotaxin-3 genes are located within a region of approximately 40 kilobases on human chromosome 7q11.23. Three variants of 73, 75, and 76 residues truncated at the C-terminus have been described also. The protein is distantly related to, but functionally very similar, to Eotaxin. Murine eotaxin-2 appears to be produced constitutively in the jejunum and spleen. Production is induced by allergen challenge and IL4 overexpression (Zimmermann et al, 2000). Eotaxin-2 induces chemotaxis of eosinophils and basophils, and the release of histamine and leukotriene C-4 from basophils primed with IL3. Eotaxin-2 activates chemotaxis-related events such as actin polymerization, and the respiratory burst almost as efficiently as Eotaxin. Monoclonal antibodies (7B11) selectively blocking the eotaxin receptor, CCR3, also block migration and release responses. Intradermal injection of eotaxin-2 in a rhesus monkey (100 or 1,000 pmol per site) induces a marked local infiltration of eosinophils. Cellular Localization: Secreted Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.