EP2 Receptor

Prostanoids are the cyclooxygenase metabolites derived from C-20 unsaturated fatty acids (arachidonic acid) and include prostaglandin (PG) D2, PGE2, PGF2 alpha, PGI2, and thrombaxane (Tx) A2. The fatty acids precursors are released from the membrane phosphoplipids in response to various physiological and pathological stimuli by the action of phospholipase A2 and are converted to various prostanoids by the sequential actions of cyclooxygenases and the respective synthases. Prostaglandin PGE2 is one of the major prostaglandin produced during inflammation. A variety of PGE2-mediated effects on vascular smooth muscle tonus, glomerular cell function, renin reledid not ase, and renal salt and water transport have been described. PGs also influence neuronal activity by modulating neurotransmitter release, sensitizing secretory fibers to noxious stimuli, or inducing fever and sleep. The actions of PGE2 are mediated by rhodopsin-type; G-protein coupled membrane receptors, termed E-prostanoid (EP) receptors or PTGERs. There are four subtypes of PGE receptors designated as EP1, EP2, EP3, and EP4 that are encoded by different genes and expressed differently in each tissue. The intracellular signaling also differs among the receptor subtypes. In general, EP receptors display a protein topology typical of GPCR-7 TM domain, an extracellular N-terminus, and a large intracellular C-terminus.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-10ug/ml using ECL. Mr ~67kD.\nELISA: 0.5-1ug/ml using control peptide (E3364-26) to coat plates at 1ug/ml \nOptimal dilutions to be determined by the researcher.\n\nControl Peptide:\nE3364-26: EP2 Receptor, Human, Control Peptide\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

E3364-27

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.5, 0.1% BSA, 40% glycerol.

P Type

Pab

Purity

Purified by affinity chromatography.

Isotype

IgG

References

1. Nemoto, K., et al. (1997) Prostaglandins 54:713. 2. Boie, Y., et al., (1997) Eur. J. Pharmacol. 340:227. 3. Narumiya, S., et al., (1995) FEBS Lett. 372:151. 4. Regan, J.W., et al., (1994) Mol. Pharamcol. 46:213. 5. Smock, T.A., et al., (1999) Gene 237:393. 6. Morath, R., et al., (1999) J. Am. Soc. Nephrol. 10:1860. 7. Narumiya, S., et al. (1999) Physiol. Rev. 79:1193-1226 (review).

Additional Info

Recognizes human EP2 Receptor. No appreciable sequence homology with EP1, EP3, EP4, or other GPCRs. Species sequence homology: rabbit 85% and canine 71%. The sequence is not conserved in other species.